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MUC5AC Antibody [45M1]

Catalog Number: orb1252729

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$ 1,070.00
Catalog Numberorb1252729
CategoryAntibodies
DescriptionMUC5AC Antibody [45M1]
TargetMUC5AC
ClonalityMonoclonal
IsotypeIgG1, kappa
ConjugationUnconjugated
ReactivityFeline, Gallus, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat
Form/AppearanceLiquid
Concentration0.2 mg/mL
Buffer/PreservativesPBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
ImmunogenM1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for this MUC5AC antibody.
UniProt IDP98088
Tested applicationsFC, IF, IHC
Application notesFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC5AC antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Antibody TypePrimary Antibody
Clone Number45M1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesMUC5AC, Gastric mucin, Major airway glycoprotein,
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NoteFor research use only
MUC5AC Antibody [45M1]

IHC testing of human stomach stained with MUC5AC antibody (45M1).

MUC5AC Antibody [45M1]

Intracellular flow cytometry staining of A549 cells using MUC5AC antibody (red) and isotype control (green).

MUC5AC Antibody [45M1]

IHC testing of human gastric carcinoma stained with MUC5AC antibody (45M1).

MUC5AC Antibody [45M1]

IHC testing of rat stomach stained with MUC5AC antibody (45M1).