MTM1 Antibody

SKU: orb1247247

Description

MTM1 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 70kDa band observed in lysates of Human and Mouse Skeletal Muscle and of cell line HepG2 (calculated MW of 69.9kDa according to NP_000243.1and 69.6kDa according to Mouse NP_001157662.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SSPSQMMPHVQTHF
• Target: MTM1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

MTM1, myotubular myopathy 1, CNM, MTMX, XLMTM, myotubularin, myotubularin 1

orb1247247 (1 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247247 (1 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247247 (1 ug/ml) staining of Mouse Skeletal Muscle lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247247 (10 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

MTM1

UniProt

Q13496

RefSeq (Protein):NP_000243.1