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MTM1 Antibody (C-term)
Catalog Number: orb1929788
| Catalog Number | orb1929788 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB0856 |
| Tested applications | WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:2000, WB: 1:1000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 69932 Da |
| Target | This MTM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 566-594 amino acids from the C-terminal region of human MTM1. |
| UniProt ID | Q13496 |
| NCBI | NP_000243.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Myotubularin, Phosphatidylinositol-3,5-bisphosphat Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot showing knockdown of endogenous MTM1 expression by MTM1-targeting vectors pDM134 and pDM170. Embryonic stem (ES) cells were untreated (lane 1) or transfected with control plasmid pDCont (lane 2), MTM1-targeting plasmid pDM134 (lane 3), or pDM170 (lane 4). The blot was probed with anti-MTM1 rabbit polyclonal antibodies. -Actin was used as a loading control.
All lanes: Anti-hMTM1-K581 at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 70 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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