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Catalog Number | orb1258119 |
---|---|
Category | Antibodies |
Description | MT1-MMP Antibody |
Target | MMP14 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Purification | Affinity purification |
Immunogen | Recombinant protein of human MT1-MMP |
UniProt ID | P50281 |
MW | Observed: 46-70kDa |
Tested applications | IHC, WB |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:100 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | MMP14, matrix metallopeptidase 14 (membrane-insert Read more... |
Note | For research use only |
Western blot analysis of extracts of various cell lines, using MT1-MMP antibody (orb1258119) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using MT1-MMP antibody (orb1258119) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using MT1-MMP antibody (orb1258119) at dilution of 1:100 (40x lens).
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |