MMP14/MT1-MMP Antibody

• Catalog Number: orb1294372
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MMP14
• Target: MMP14 / MT1-MMP
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human MMP14 / MT1-MMP
• Tested applications: IF, IHC, WB
• Dilution range: Western Blot 1:500 Immunofluorescence 1:150-1:300 Immunohistochemistry (Paraffin) 1:100-1:200
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-MMP14 / MT1-MMP antibody - Loading Control at 1/500 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with orb1294372 overnight at 4°C.

All lanes: Anti-MMP14 / MT1-MMP antibody - Loading Control at 1/500 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with orb1294372 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling MMP14 / MT1-MMP with orb1294372 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling MMP14 / MT1-MMP with orb1294372 at 1/100.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294372 anti-MMP14 / MT1-MMP antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294372 anti-MMP14 / MT1-MMP antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling MMP14 / MT1-MMP with orb1294372 at 1/100.