MSI2 / musashi-2 Antibody

• Catalog Number: orb1248128
• Category: Antibodies
• Description: MSI2 / musashi-2 Antibody
• Target: MSI2 / musashi-2
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-SPDSLRDYFSK
• UniProt ID: Q96DH6
• MW: Approx 37kDa
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in lysates of cell line Kelly (calculated MW of 35.2kDa according to NP_620412.1). Recommended concentration: 0.1-0.3ug/ml. Approx 37+39kDa bands observed in fetal Mouse Brain lysates (calculated MW of 36.9kDa according to Mouse NP_473384.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MSI2, musashi RNA-binding protein 2, MSI2H, RNA-bi
• Note: For research use only
• NCBI: NP_733839.1, NP_620412.1

orb1248128 (0.1 ug/ml) staining of Kelly lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248128 (0.3 ug/ml) staining of fetal Mouse Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248128 (5 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.