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MSI2/musashi-2 (aa33-43) Antibody

SKU: orb195345

Description

Goat polyclonal antibody to MSI2

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman, Mouse
Predicted ReactivityBovine
Application Notes
This antibody is expected to recognize both reported isoforms (NP_620412.1; NP_733839.1).ELISA: antibody detection limit dilution 1:128000.WB: Approx 37kDa band observed in lysates of cell line Kelly (calculated MW of 35.2kDa according to NP_620412.1). Recommended concentration: 0.1-0.3µg/ml. Approx 37+39kDa bands observed in fetal Mouse Brain lysates (calculated MW of 36.9kDa according to Mouse NP_473384.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1µg/ml.

Key Properties

ClonalityPolyclonal
TargetMSI2 / musashi-2 (aa33-43)
Protein SequenceSPDSLRDYFSK
Molecular Weight35.2; 28.4
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti MSI2 antibody, anti musashi RNA-binding protein 2 antibody, anti MSI2H antibody, anti RNA-binding protein Musashi homolog 2 antibody, anti musashi homolog 2 antibody, anti musashi-2 antibody
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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC
Immunohistochemistry
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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