Mouse TNF protein

SKU: orb392581

Description

Recombinant mouse TNF protein is produced by our E. coli expression system and the target gene encoding Asp89-Leu235 is expressed.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: TNF
• Reactivity: Mouse
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: MDKPVAHVVA NHQVEEQLEW LSQRANALLA NGMDLKDNQL VVPADGLYLV YSQVLFKGQG CPDYVLLTHT VSRFAISYQE KVNLLSAVKS PCPKDTPEGA ELKPWYEPIY LGGVFQLEKG DQLSAEVNLP KYLDFAESGQ VYFGVIAL

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μM filtered solution of PBS, pH 7.4.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Quick Database Links

Gene Symbol

TNF

UniProt

P06804

NCBI Gene ID

21926