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Catalog Number | orb392581 |
---|---|
Category | Proteins |
Description | Recombinant mouse TNF protein is produced by our E. coli expression system and the target gene encoding Asp89-Leu235 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Mouse |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | TNF |
Entrez | 21926 |
UniProt ID | P06804 |
Protein Sequence | MDKPVAHVVA NHQVEEQLEW LSQRANALLA NGMDLKDNQL VVPADGLYLV YSQVLFKGQG CPDYVLLTHT VSRFAISYQE KVNLLSAVKS PCPKDTPEGA ELKPWYEPIY LGGVFQLEKG DQLSAEVNLP KYLDFAESGQ VYFGVIAL |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Alternative names | TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TN Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 98% as determined by SDS-PAGE and HPLC. | |
17.4 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
16.4 kDa | |
E.coli |
Unconjugated | |
90% | |
14.6 kDa | |
Mouse GDF-15, His Tag (orb1496172) is expressed from E. coli cells. It contains AA Ser 189 - Ala 303 (Accession # Q9Z0J7-1). |
Unconjugated | |
95% | |
19.1 kDa | |
Mouse TNF-alpha, His Tag (orb1496307) is expressed from human 293 cells (HEK293). It contains AA Leu 80 - Leu 235 (Accession # P06804). |
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