CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes) Antibody

SKU: orb1410562

Description

Mouse monoclonal antibody to CD40 Ligand

Images & Validation

• Tested Applications: IHC
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: CD40LG/4675
• Immunogen: Recombinant fragment (around aa 108-261) of human CD40LG protein(exact sequence is proprietary)
• Target: CD40LG
• Source/Expression System: Bioreactor
• Molecular Weight: 36kDa
• Purification: Protein G

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Disclaimer: For research use only

Alternative Names

CD154; gp39; hCD40L; HIGM1; IGM; IMD3; T B cell activating molecule; T BAM; T-cell antigen Gp39; TNF-related activation protein; TrAP; Tumor necrosis factor ligand superfamily member 5 (TNFSF5)

Analysis of Protein Array containing more than 19000 full-length human proteins using CD40-Ligand Mouse Monoclonal Antibody (CD40LG/4675). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human kidney stained with CD40L-Monospecific Mouse Monoclonal Antibody (D40LG/4675) at 2 ug/ml. HIER: Tris/EDTA, pH9.0, 45 min. 2°C: HRP-polymer, 30 min. DAB, 5 min.

Formalin-fixed, paraffin-embedded human liver carcinoma in colon stained with CD40L-Monospecific Mouse Monoclonal Antibody (CD40LG/4675) at 2 ug/ml. HIER: Tris/EDTA, pH9.0, 45 min. 2°C: HRP-polymer, 30 min. DAB, 5 min.

SDS-PAGE Analysis of Purified CD40L-Monospecific Mouse Monoclonal Antibody (CD40LG/4675). Confirmation of Purity and Integrity of Antibody.

Quick Database Links

Gene Symbol

CD40LG

UniProt

P29965