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(Mouse) Shh Antibody (C-term)
Catalog Number: orb1788138
| Catalog Number | orb1788138 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC-P, WB |
| Reactivity | Mouse, Rat |
| Isotype | Rabbit IgG |
| Immunogen | 397-431 aa |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, IHC-P: 1:25 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 47773 Da |
| Target | This (Mouse) Shh antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 397-431 amino acids from the C-terminal region of Mouse Shh. |
| UniProt ID | Q62226 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Sonic hedgehog protein, SHH, HHG-1, Sonic hedgehog Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded M. lung section using (Mouse) Shh Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
All lanes: Anti-Shh Antibody (C-term) at 1/1000 dilution. Lane 1: mouse liver lysates. Lane 2: mouse lung lysates. Lane 3: mouse stomach lysates. Lane 4: rat lung lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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