Mouse Mapk3 Antibody (C-term)

• Catalog Number: orb1935974
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB34827
• Tested applications: FC, IHC-P, WB
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 43066 Da
• Target: This Mouse Mapk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-380 amino acids from the C-terminal region of mouse Mapk3.
• UniProt ID: Q63844
• NCBI: NP_036082.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Mitogen-activated protein kinase 3, MAP kinase 3,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Hela cells using Mouse Mapk3 Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysates from mouse BA/F3, C2C12, NIH/3T3 and rat C6 cell line (from left to right), using Mouse Mapk3 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Mouse Mapk3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of Mouse Mapk3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.