Mouse Mapk3 Antibody (C-term)

SKU: orb1788452

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:25
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This Mouse Mapk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-380 amino acids from the C-terminal region of mouse Mapk3. Antigen Region: 353-380 aa.
• Target: Mapk3
• Molecular Weight: 43066 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mapk3; Erk1; Prkm3; Mitogen-activated protein kinase 3; ERT2; Extracellular signal-regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; MNK1; Microtubule-associated protein 2 kinase; p44-ERK1

Flow cytometric analysis of Hela cells using Mouse Mapk3 Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysates from mouse NIH/3T3, BA/F3, mouse C2C12, rat C6 cell line (from left to right), using Mouse Mapk3 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Mouse Mapk3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of Mouse Mapk3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

Mapk3

UniProt

Q63844

RefSeq (Protein):NP_036082.1