Mouse Lyn Antibody (Center)

• Catalog Number: orb1926981
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB50588
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 58812 Da
• Target: This Mouse Lyn antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 229-263 amino acids from the Central region of human Mouse Lyn.
• UniProt ID: P25911
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Tyrosine-protein kinase Lyn, V-yes-1 Yamaguchi sar
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded M.spleen section using Mouse Lyn Antibody (Center). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from Raji cell line and rat spleen tissue lysate (from left to right), using Mouse Lyn Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

All lanes: Anti-Lyn Antibody (Center) at 1:2000 dilution. Lane 1: mouse kidney lysates. Lane 2: mouse heart lysates. Lane 3: mouse ovary lysates. Lane 4: mouse skeletal muscle lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.