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Catalog Number | orb1788145 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC-P, WB |
Predicted Reactivity | Mouse, Rat |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | 205-239 aa |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:25 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 58574 Da |
Target | This LYN antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 205-239 amino acids from the Central region of human LYN. |
UniProt ID | P07948 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Tyrosine-protein kinase Lyn, Lck/Yes-related novel Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded H. liver section using LYN Antibody (Center). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
All lanes: Anti-LYN Antibody (Center) at 1/1000 dilution. Lane 1: K562 whole cell lysates. Lane 2: Raji whole cell lysates. Lane 3: Ramos whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |