Mouse IgG2a Antibody

SKU: orb347527

Description

MOUSE IgG2a antibody

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution Range: ELISA: 1:15,000 - 1:30,000, IHC: 1:2,000 - 1:10,000, WB: 1:4,000 - 1:20,000
• Reactivity: Mouse
• Application Notes:
  Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Mouse IgG2a heavy chain
• Purity: Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum, Mouse IgG and Mouse IgG2a. Specificity was confirmed by ELISA. For IgG1, IgG2b and IgG3 reactivity was less than 5% against target (IgG2a, IgG).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.02 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-Mouse IgG2a Antibody, Mouse IgG2a Secondary Antibody, Rb-a-mouse IgG2a

Dot Blot of Rabbit Anti-Mouse IgG2a Antibody. Tested with dilutions 10 ng, 3.3 ng, 1.1 ng, 0.37 ng, 0.12 ng. Lane 1: Mouse IgG1 (p/n orb2652749). Lane 2: Mouse IgG2a (p/n orb343777). Lane 3: Mouse IgGb (p/n orb343778). Lane 4: Mouse IgG3 (p/n orb343779). Lane 5: Mouse IgM (p/n orb346283). Primary Antibody: Rabbit anti-Mouse IgG2a at 1 µg/ml for 1 hour at RT. Secondary Antibody: Gt-anti-Rabbit HRP (p/n orb347654) at 1:40000. Blocking Buffer: BlockOut orb348644 for 30 min at RT.

Western Blot of Rabbit anti-Mouse IgG2a antibody. Lane 1: Mouse IgG1 (p/n orb2652749). Lane 2: Mouse IgG2a (p/n orb343777). Lane 3: Mouse IgG2b (p/n orb343778). Lane 4: Mouse IgG3 (p/n orb343779). Lane 5: Mouse IgM (p/n orb346283). Load: 5 ng per lane. Primary antibody: Mouse IgG2a antibody at 1 µg/ml for overnight at 4°C. Secondary antibody: Gt-anti-Rabbit HRP (p/n orb347654) at 1:40000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 50 kDa and25 kDa, 50 kDa and 25 kDa for Mouse IgG2a. Other band(s): Weak reactivity for to Mouse IgG1.