FKBP52 Antibody

• Catalog Number: orb1822486
• Category: Antibodies
• Description: Mouse Anti-Human FKBP52 Monoclonal IgG2a
• Target: FKBP52
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a
• Conjugation: Unconjugated
• Reactivity: Canine, Hamster, Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Immunogen: Synthetic peptide corresponding to the residues of human FKBP52
• UniProt ID: Q02790
• MW: 52kDa
• Tested applications: ICC, IF, IHC, IP, WB
• Dilution range: WB (1:2000), IHC (1:250), ICC/IF (1:1000), IP (5µg); optimal dilutions for assays should be determined by the user.
• Application notes: 0.5 µg/ml was sufficient for detection of FKBP52 in 20 µg total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
• Clone Number: Hi52C
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FK506 binding protein 4 Antibody, FKBP4 Antibody,
• Note: For research use only
• Entrez: 2288
• NCBI: NP_002005.1
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Tissue: prostate tissue (ductal epithelial cells). Species: Human. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1:1000.

Immunohistochemistry analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Western Blot analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1.5 μg/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Western Blot analysis of Human Cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1.5 μg/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Tissue: MCF-7 cells (metastatic mammary gland/breast cell line). Species: Human. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red).