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Catalog Number | orb348520 |
---|---|
Category | Antibodies |
Description | Mouse IgG IgA IgM (H&L) Pre-Adsorbed Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | DOT, ELISA, IHC, WB |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | Mouse IgG IgA and IgM whole molecule |
Antibody Type | Secondary Antibody |
Concentration | 1.2 mg/mL |
Dilution range | ELISA: 1:50,000-1:90,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Secondary Antibody was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by cross adsorption to remove unwanted specificities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins. This reagent is suitable for the detection of all mouse isotypes and chain combinations. ELISA was used to confirm specificity at less than 1% of the target signal. |
Conjugation | Unconjugated |
Storage | Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Goat Anti Mouse IgG IgA IgM Pre-Adsorbed, Goat Ant Read more... |
Note | For research use only |
Application notes | Anti-Mouse IgG IgA IgM antibody has been tested by ELISA, SDS-PAGE, and Dot Blot, and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. |
Expiration Date | 12 months from date of receipt. |
ELISA, IHC, WB | |
Mouse | |
Goat | |
Polyclonal | |
HRP |
FC, FLISA, IF | |
Mouse | |
Goat | |
Polyclonal | |
TRITC |
FC, FLISA, IF | |
Mouse | |
Goat | |
Polyclonal | |
FITC |
ELISA, IHC, WB | |
Mouse | |
Goat | |
Polyclonal | |
Biotin |
FC, FLISA, IF | |
Mouse | |
Goat | |
Polyclonal | |
Texas Red |