Mouse IgG IgA IgM (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

SKU: orb347388

Description

Mouse IgG IgA IgM (H&L) antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Reactivity: Mouse
• Application Notes:
  Anti-Mouse IgG IgA IgM Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Mouse IgG IgA and IgM whole molecule
• Purity: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against bovine, horse or human serum proteins. This reagent is suitable for the detection of all Mouse immunoglobulin isotypes and chain combinations.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Goat anti-mouse IgG IgA IgM antibody peroxidase conjugation, goat anti-mouse IgGAM HRP conjugated antibody

ELISA results of purified Goat Anti-MOUSE IgG IgA IgM Peroxidase Antibody (Min X Bv, Hs, Hu Serum Proteins) tested against purified MOUSE IgG IgA IgM. Each well was coated in duplicate with 1.0 µg of MOUSE IgG (red line) IgA (green line) IgM (blue line). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.