Mouse Human IgE (class specific) Antibody

SKU: orb22206

Description

Monoclonal mouse antibody to Human IgE, isotype specific. To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno- peroxidase and immunofluorescence staining of cytoplasmic IgE and immunoblotting. The optimum working dilution is an assay- related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100; in ELISA from 1:50 upwards; in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only. Working dilutions may vary widely, strongly depending on the test conditions.

Images & Validation

• Reactivity: Human

Key Properties

• Antibody Type: Secondary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: NI 307
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/ml. No foreign proteins added.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only