Mouse Human IgE (class specific), conjugated with Horseradish peroxidase Antibody

SKU: orb22245

Description

Horseradish peroxidase conjugated purified monoclonal mouse antibody to Human IgE, isotype specific. To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:250; in ELISA from 1:250 upwards; in Western blotting from 1:500 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Images & Validation

• Tested Applications: DOT, ELISA, ICC, IHC, WB
• Reactivity: Human

Key Properties

• Antibody Type: Secondary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: NI 307
• Conjugation: HRP

Storage & Handling

• Storage: The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only