Mouse anti Human CD45

• Catalog Number: orb1570850
• Category: Antibodies
• Description: Mouse anti Human CD45
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BHPT-1
• Tested applications: FC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient
• Concentration: See vial for concentration
• Form/Appearance: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
• UniProt ID: P08575
• Storage: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
• Buffer/Preservatives: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Alternative names: CD45
• Note: For research use only
• Application notes: PBMC: Add 10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20ºC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5+: As above, however use only 5 µl per test rather than 10 µl. Excites at 488nm and emits at 670nm. Store protected from light.
• Expiration Date: 12 months from date of receipt.