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Product Overview
| Product Name | CD45 antibody |
|---|---|
| Catalog Number | orb10328 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Porcine, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, P-ELISA, WB |
| Immunogen | KLH conjugated synthetic peptide derived from human CD45. Please contact us for the exact immunogen sequence. The peptide is available as orb374714. |
| Target | CD45 |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
|---|---|
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml - 200 μg (in 400 μl): 0.5 mg/ml |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| MW | 143 kDa |
| Uniprot ID | P08575 (Human); P06800 (Mouse); P04157 (Rat) |
| NCBI | NP_002829.31; NM_002838.41 |
| Entrez | 5788 |
Product Description
Rabbit polyclonal antibody to CD45
Application Notes
| Dilution Range | IF/ICC: 1:100-500, WB: 1:200-1000, IHC-P: 1:100-500 (based on 0.5 mg/ml) |
|---|
Validation Images 
Immunohistochemical staining of mouse skin tissue using CD45 antibody (dilution of primary antibody - 1:100)
WB analysis of rat pancreas (lane 1), rat large intestines (lane 2), rat heart (lane 3), human breast cancer (lane 4) using CD45 antibody (1 ug/ml)
Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma using CD45 antibody.
Immunofluorescence image of mouse skin tissue using CD45 antibody (dilution at 1:100)
IF analysis of mouse skin tissue using anti-CD45 (dilution of primary antibody at 1:100)
IHC-P image of pig uterus tissue using anti-CD45 (2.5 ug/ml)
Immunofluorescence image of mouse lung tissue using anti-CD45 (dilution at 1:100)
Immunohistochemical staining of pig lung tissue using CD45 antibody (2.5 ug/ml)
IHC-P image of mouse lung tissue using CD45 antibody (dilution of primary antibody at 1:100)
Immunofluorescence analysis of mouse lung tissue using anti-CD45 (dilution of primary antibody - 1:100)
Immunohistochemical staining of mouse kidney tissue using CD45 antibody (dilution of primary antibody - 1:100)
Immunofluorescence analysis of mouse kidney tissue using anti-CD45 (dilution of primary antibody - 1:100)
Immunohistochemical staining of mouse lung tissue using CD45 antibody (dilution of primary antibody - 1:100)
Western blot analysis of mouse spleen tissue using CD45 antibody(Dilution at 1:500)
IHC-P staining of mouse skin tissue using anti-CD45 (dilution at 1:100)
WB analysis of mouse liver (lane 1), rat lung (lane 2), rat uterus (lane 3), rat spleen (lane 4) using CD45 antibody (1 ug/ml)
Immunohistochemical analysis of paraffin-embedded rat brain tissue using CD45 antibody.
Immunofluorescence image of rat lung tissue using Reelin antibody (2.5 ug/ml)
IF analysis of rat lung tissue using Reelin antibody (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat spleen tissue using anti-CD45 (2.5 ug/ml)
Immunohistochemical staining of mouse skin tissue using anti-CD45 (2.5 ug/ml)
Immunohistochemical staining of rat spleen tissue using anti-CD45 (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded mouse skin tissue using CD45 antibody (2.5 ug/ml)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD45 antibody.
Immunohistochemical staining of mouse skin tissue using anti-CD45 (dilution of primary antibody - 1:100)
Western blot analysis of mouse liver (lane 1), rat lung (lane 2), rat uterus (lane 3), rat spleen (lane 4) using CD45 antibody (1 ug/ml)
IHC-P image of mouse skin tissue using CD45 antibody (dilution of primary antibody at 1:100)
Western blot analysis of rat large intestines (lane 1), rat heart (lane 2), human breast cancer (lane 3) using CD45 antibody (1 ug/ml)
Immunohistochemical staining of paraffin embedded mouse skin tissue using anti-CD45 (primary antibody at 1:100)
Immunohistochemical staining of paraffin embedded pig lung tissue using anti-CD45 (2.5 ug/ml)
IHC-P staining of pig uterus tissue using CD45 antibody (2.5 ug/ml)
IHC-P staining of pig spleen tissue using CD45 antibody (2.5 ug/ml)
Immunohistochemical staining of pig heart tissue using CD45 antibody (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded pig spleen tissue using anti-CD45 (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded mouse lung tissue using CD45 antibody (primary antibody at 1:100)
Immunohistochemical staining of pig uterus tissue using CD45 antibody (2.5 ug/ml)
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