Mouse anti Human CD19

• Catalog Number: orb1570876
• Category: Antibodies
• Description: Mouse anti Human CD19
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1G9
• Tested applications: FC
• Reactivity: Human
• Isotype: IgG1
• Concentration: See vial for concentration
• Form/Appearance: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
• UniProt ID: P15391
• Storage: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
• Buffer/Preservatives: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Alternative names: CD19
• Note: For research use only
• Application notes: PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8º C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20º C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5+: Excites at 488nm and emits at 670nm. Store protected from light.
• Expiration Date: 12 months from date of receipt.