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CD19 antibody
Description
Images & Validation
−| Tested Applications | ELISA, ICC, IF, IHC-P, WB |
|---|---|
| Dilution range | WB: 1:200-2000, IF/ICC: 1:50-300, IHC-P: 1:100-500 (based on 0.5 mg/ml) |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human CD19. Please contact us for the exact immunogen sequence. The peptide is available as orb374859. |
| Target | CD19 |
| Molecular Weight | 128 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of rat lymph node (lane 1), rat spleen (lane 2), rat lung (lane 3), mouse brain (lane 4), human breast cancer (lane 5), human ovarian cancer (lane 6) using CD19 antibody (1 ug/ml)

Immunofluorescence analysis of rat lymph node tissue using anti-CD19 (2.5 ug/ml)

IHC-P staining of rat brain tissue using CD19 antibody (dilution at 2.5 ug/ml)

IHC-P staining of mouse brain tissue using anti-CD19 (dilution at 2.5 ug/ml)

WB analysis of mouse lymph node (lane 1), mouse spleen (lane 2), mouse brain (lane 3); mouse liver (lane 4) using CD19 antbody (1 ug/ml)

IHC-P image of rat thymus tissue using CD19 antibody (dilution of primary antibody at 2.5 ug/ml)

IHC-P staining of rat brain tissue using anti-CD19 (dilution at 2.5 ug/ml)

WB analysis of rat lymph node (lane 1), rat spleen (lane 2), rat lung (lane 3), mouse brain (lane 4), human breast cancer (lane 5), human ovarian cancer (lane 6) using CD19 antibody (1 ug/ml)

IF image of rat lymph node tissue using CD19 antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse lymph node tissue using anti-CD19 (primary antibody at 2.5 ug/ml)
Quick Database Links
UniProt Details
−NCBI Gene Details
−NCBI Reference Sequences
−| mRNA | NM_001770.51, NM_001770.5 |
|---|---|
| Protein | NP_001761.31, NP_001761.3 |
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Protocol Information
CD19 antibody (orb10306)
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