Mouse anti-Cytokeratin Monoclonal Antibody

• Catalog Number: orb1520125
• Category: Antibodies
• Description: Cytokeratin Antibody
• Target: Cytokeratin
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 200 µg/ml
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Purity: The antibody was purified from cell culture supernatant
• Immunogen: Human epidermal callus
• UniProt ID: P13645
• Tested applications: ICC, IF, IHC
• Dilution range: Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. ICC-IF1:100 - 1:500. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Multiplex Immunofluorescence (mIF) 1:250
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Clone Number: AE1/AE3
• Storage: 2 - 8°C
• Alternative names: K10; Keratin-10; keratin, type I cytoskeletal 10;
• Note: For research use only
• NCBI: NP_005548, NP_002266, NP_002267, NP_000412, NP_000517

Detection of human Cytokeratin by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Mouse anti-Cytokeratin monoclonal antibody [AE1/AE3]. Secondary: HRP-conjugated goat anti-mouse IgG.

Detection of human Cytokeratin by immunocytochemistry. Sample: FFPE section of MCF7 cells. Antibody: Mouse anti-Cytokeratin monoclonal antibody [AE1/AE3] (orb1520125). Secondary: HRP-conjugated goat anti-mouse IgG.

Detection of human Cytokeratin by immunocytochemistry. Sample: acetone/methanol fixed Hela cells. Antibody: Mouse monoclonal anti-Cytokeratin antibody [AE1/AE1] (orb1520125) used at 1:100. Secondary: DyLight® 594-conjugated goat anti-mouse IgG. Counterstain: DAPI.

Detection of human Cytokeratin (green) by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Mouse anti-Cytokeratin monoclonal antibody used at 1:500. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: Opal™. Counterstain: DAPI (blue).