Cytokeratin Mouse Monoclonal Antibody

SKU: orb1520125

Description

Cytokeratin Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ICC, IF, IHC
• Dilution Range: Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. ICC-IF1:100 - 1:500. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Multiplex Immunofluorescence (mIF) 1:250
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: AE1/AE3
• Immunogen: Human epidermal callus
• Target: Cytokeratin
• Purity: The antibody was purified from cell culture supernatant
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BCIE; BIE; CK-10; CK10; Cytokeratin-10; EHK; K10; KPP; Keratin-10; cytokeratin 10; keratin 10, type I; keratin, type I cytoskeletal 10

Detection of human Cytokeratin by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Mouse anti-Cytokeratin monoclonal antibody [AE1/AE3]. Secondary: HRP-conjugated goat anti-mouse IgG.

Detection of human Cytokeratin by immunocytochemistry. Sample: FFPE section of MCF7 cells. Antibody: Mouse anti-Cytokeratin monoclonal antibody [AE1/AE3] (orb1520125). Secondary: HRP-conjugated goat anti-mouse IgG.

Detection of human Cytokeratin by immunocytochemistry. Sample: acetone/methanol fixed Hela cells. Antibody: Mouse monoclonal anti-Cytokeratin antibody [AE1/AE1] (orb1520125) used at 1:100. Secondary: DyLight® 594-conjugated goat anti-mouse IgG. Counterstain: DAPI.

Detection of human Cytokeratin (green) by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Mouse anti-Cytokeratin monoclonal antibody used at 1:500. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Quick Database Links

Gene Symbol

Cytokeratin

UniProt

P13645, P19012, P08727, P08779, P02533

RefSeq (Protein):NP_000412.4