Monocrotaline

SKU: orb1225330

Description

Extracted from Legume Crotalaria assamica;Suitability:Methanol,ethanol,chloroform;Store the product in sealed,cool and dry condition.(In Vitro):Monocrotaline (MCT) is an 11-membered macrocyclic pyrrolizidine alkaloid (PA) derived from the seeds of the Crotalaria pallida plant. Monocrotaline a natural ligand exhibits dose-dependent cytotoxicity with potent antineoplastic activity. The in vitro cytotoxicity of monocrotaline is proved at IC50 24.966 μg/mL and genotoxicity at 2 X IC50 against HepG2 cells.\n(In Vivo):MCT causes a pulmonary vascular syndrome in rats characterized by proliferative pulmonary vasculitis, pulmonary hypertension (PH), and cor pulmonale. Among preclinical models of pulmonary arterial hypertension (PAH), monocrotaline animal model offers the advantage of mimic several key aspects of human PAH, including vascular remodeling, proliferation of smooth muscle cells, endothelial dysfunction, upregulation of inflammatory cytokines, and right ventricle failure, requiring a single drug injection. Changes in multiple pathways associated with the development of PH, including activated glycolysis, increased markers of proliferation, disruptions in carnitine homeostasis, increased inflammatory and fibrosis biomarkers, and a reduction in glutathione biosynthesis are observed with the injection of monocrotaline.

Key Properties

• CAS Number: 315-22-0
• MW: 325.36
• Purity: >98% (HPLC)
• Formula: C₁₆H₂₃NO₆
• SMILES: O=C(O[C@]1([H])CCN2[C@]1([H])C(CO3)=CC2)[C@H](C)[C@@](C)(O)[C@@](C)(O)C3=O
• Target: TGFBR
• Solubility: DMSO: 10 mM

Bioactivity

• In Vitro:
  Cell Viability Assay Cell line: HepG2 cells. Concentration: 25, 50, 100 and 200 μg/mL. Incubation time: 48 h. Result: Induced apoptosis rate was dose-dependent.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Crotaline | MCT | NSC 28693

Quick Database Links

Gene Symbol

TGFBR