MMP9 Antibody

SKU: orb1294373

Description

Rabbit polyclonal antibody to MMP 9

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: Western Blot 1:500 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:200
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Target: MMP9
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Disclaimer: For research use only

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294373 anti-MMP9 antibody (red) at 1:250 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling MMP9 with orb1294373 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Colon cancer tissue labeling MMP9 with orb1294373 at 1/100.

Immunohistochemical analysis of paraffin embedded Human kidney cancer tissue labeling MMP9 with orb1294373 at 1/100.

Immunohistochemical analysis of paraffin embedded Human kidney cancer tissue labeling MMP9 with orb1294373 at 1/100.

MMP9 antibody - at 1/500 dilution. Lysates at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294373 overnight at 4°C.

MMP9 antibody - at 1/500 dilution. Lysates at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294373 overnight at 4°C.

Quick Database Links

Gene Symbol

MMP9