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Anti-MMP9 Antibody

Catalog Number: orb315176

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb315176
CategoryAntibodies
DescriptionAnti-MMP9 Antibody. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IHC, WB
ReactivityHuman
IsotypeRabbit IgG
ImmunogenA synthetic peptide corresponding to a sequence at the C-terminus of human MMP-9, different from the related mouse sequence by fourteen amino acids, and from the related rat sequence by sixteen amino acids.
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW79 kDa
UniProt IDP14780
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesMatrix metalloproteinase-9; MMP-9; 3.4.24.35; 92 k
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NoteFor research use only
Application notesWestern blot, 0.1-0.5μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human ELISA, 0.1-0.5μg/ml, -,. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-MMP9 Antibody

IHC analysis of MMP9 using anti-MMP9 antibody. MMP9 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-MMP9 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-MMP9 Antibody

IHC analysis of MMP9 using anti-MMP9 antibody. MMP9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-MMP9 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-MMP9 Antibody

IHC analysis of MMP9 using anti-MMP9 antibody. MMP9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-MMP9 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-MMP9 Antibody

IHC analysis of MMP9 using anti-MMP9 antibody. MMP9 was detected in a paraffin-embedded section of human lymph nodes of gastric adenocarcinoma rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-MMP9 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-MMP9 Antibody

IHC analysis of MMP9 using anti-MMP9 antibody. MMP9 was detected in a paraffin-embedded section of human lymphadenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-MMP9 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-MMP9 Antibody

Western blot analysis of MMP9 using anti-MMP9 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP9 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MMP9 at approximately 79 kDa. The expected band size for MMP9 is at 79 kDa.

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    IF,  IH,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    30 μl, 100 μl, 200 μl
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    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
  • Anti-MMP9 Antibody [orb1289729]

    IF,  IH,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • Anti-MMP9 Rabbit Polyclonal Antibody [orb2569364]

    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
  • Anti-MMP9 Rabbit Polyclonal Antibody [orb2569365]

    IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μg