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Catalog Number | orb1294376 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to MMP 1 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Rat |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the center region |
Dilution range | Western Blot 1:500 Immunofluorescence 1: 100- 1:300 Immunohistochemistry (Paraffin) 1: 100-1:200 |
Conjugation | Unconjugated |
Target | MMP1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-MMP1 antibody at 1/500 dilution, Lysates/proteins at 40 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294376 overnight at 4°C.
All lanes: Anti-MMP1 antibody at 1/500 dilution, Lysates/proteins at 40 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294376 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294376 anti-MMP1 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294376 anti-MMP1 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded mouse testis labeling MMP1 with orb1294376 at 1/100.
IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Equine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Animal, Human | |
Equine, Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |