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Catalog Number | orb1256869 |
---|---|
Category | Antibodies |
Description | MLPH Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 301-600 of human MLPH (NP_077006.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 75kDa |
Target | MLPH |
UniProt ID | Q9BV36 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Melanophilin, Exophilin-3, Slp homolog lacking C2 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of Mouse stomach, using MLPH antibody (orb1256869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunofluorescence analysis of NIH-3T3 cells using MLPH antibody (orb1256869) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using MLPH antibody (orb1256869) at dilution of 1:100. Blue: DAPI for nuclear staining.
ELISA, ICC, IF, Pull-down Assay, WB | |
Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, Pull-down Assay, WB | |
Rat | |
Mouse | |
Goat | |
Polyclonal | |
Unconjugated |
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