Melanophilin (mouse) Antibody

• Catalog Number: orb18700
• Category: Antibodies
• Description: Goat polyclonal antibody to Mlph
• Target: Melanophilin (mouse)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ARHIFAKPVMAQQP
• RRID: AB_10754199
• MW: 65
• Tested applications: ELISA, ICC, IF, Pull-down Assay, WB
• Dilution range: ELISA: 1:32000, WB: 0.5-2ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 80 kDa band observed in Mouse Melanocyte extracts. Recommended for use at 0.5-2 μg/ml. Please note that this product may require overnight incubation of primary and high protein loads.Experiment Notes: Immunofluorescence: successfully used at 0.5ug/ml to stain mouse melanocytes, see picture. Immunofluorescence: see also publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. Epub 2008 Jun 17. (PMID:18559336). GST-pull down assay: see publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. Epub 2008 Jun 17. (PMID:18559336).
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti 2210418F23Rik antibody, anti melanophilin ant
• Research Area: Neuroscience
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of using Mlph antibody

Immunofluorescence analysis of using Mlph antibody