Melanophilin (mouse) Antibody

SKU: orb18700

Description

Goat polyclonal antibody to Mlph

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, ICC, IF, Pull-down Assay, WB
• Dilution Range: ELISA: 1:32000, WB: 0.5-2ug/ml
• Reactivity: Mouse
• Predicted Reactivity: Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 80 kDa band observed in Mouse Melanocyte extracts. Recommended for use at 0.5-2 μg/ml. Please note that this product may require overnight incubation of primary and high protein loads.Experiment Notes: Immunofluorescence: successfully used at 0.5ug/ml to stain mouse melanocytes, see picture. Immunofluorescence: see also publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. Epub 2008 Jun 17. (PMID:18559336). GST-pull down assay: see publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. Epub 2008 Jun 17. (PMID:18559336).

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Melanophilin (mouse)
• Protein Sequence: ARHIFAKPVMAQQP
• Molecular Weight: 65
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti 2210418F23Rik antibody, anti melanophilin antibody, anti Mlph antibody, anti ln antibody, anti l1Rk3 antibody, anti Slac-2a antibody, anti D1Wsu84e antibody, anti l(1)-3Rk antibody, anti 5031433I09Rik antibody, anti leaden antibody, anti AW228792 antibody, anti OTTMUSP00000025451 antibody

Western blot analysis of using Mlph antibody

Immunofluorescence analysis of using Mlph antibody

Quick Database Links

Gene Symbol

Melanophilin (mouse)