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Catalog Number | orb18700 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to Mlph |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, Pull-down Assay, WB |
Reactivity | Mouse, Rat |
Dilution range | ELISA: 1:32000, WB: 0.5-2ug/ml |
Conjugation | Unconjugated |
MW | 65 |
Target | Melanophilin (mouse) |
Protein Sequence | ARHIFAKPVMAQQP |
RRID | AB_10754199 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti 2210418F23Rik antibody, anti melanophilin ant Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 80 kDa band observed in Mouse Melanocyte extracts. Recommended for use at 0.5-2 μg/ml. Please note that this product may require overnight incubation of primary and high protein loads.Experiment Notes: Immunofluorescence: successfully used at 0.5ug/ml to stain mouse melanocytes, see picture. Immunofluorescence: see also publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. Epub 2008 Jun 17. (PMID:18559336). GST-pull down assay: see publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. Epub 2008 Jun 17. (PMID:18559336). |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of using Mlph antibody
Immunofluorescence analysis of using Mlph antibody