CENPU Antibody

SKU: orb345541

Description

MLF1 antibody

Images & Validation

• Tested Applications: ELISA, KO/KD Validated, WB
• Dilution Range: ELISA: 1:5,000 - 1:25,000, WB: 1:500- 1:2,000
• Reactivity: Bovine, Canine, Human, Primate
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 65 kDa in size corresponding to MLF1IP protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr78 of human MLF1IP protein.
• Target: CENPU
• Purity: This affinity purified antibody is directed against human MLF1IP protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with MLF1IP protein from human, dog, bovine and chimpanzee based on 100% homology with the immunizing sequence. Expect partial reactivity with homologues from rat and mouse (90% homology). Reactivity against homologues from other sources is not known. This antibody reacts with MLF1IP protein that is either phosphorylated or non-phosphorylated at Thr78.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.06 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-MLF1 antibody, rabbit anti-MLF1 Interacting Protein antibody, Centromere protein U, CENP-U, Centromere protein of 50 kDa, CENP-50, Interphase centromere complex protein 24, KSHV latent nuclear antigen-interacting protein 1, MLF1-interacting protein, Polo-box-interacting protein 1, ICEN24, PBIP1, KLIP1

Western blot using Biorbyt's affinity purified anti-MLF1IP antibody shows detection of MLF1IP (arrowhead) in HeLa cells transfected with ZZ-tagged MLF1IP (Lane 1). Lane 2 is lysate from non-transfected HeLa cells, and Lane 3 is lysate from HeLa cells containing a knock-out mutation for PBIP1/MLF1IP.

Quick Database Links

Gene Symbol

CENPU

UniProt

Q71F23

RefSeq:38016935