Mlf1 (phospho-T78) antibody

• Catalog Number: orb345549
• Category: Antibodies
• Description: Mlf1 (phospho-T78) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr78 of human MLF1IP protein. The immunogen peptide is phosphorylated at Thr78.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:25,000, IHC: 20 µg/ml, IF: User Optimized, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against the non-phospho peptide. This antibody is specific for human MLF1IP protein phosphorylated at Thr78. A BLAST analysis was used to suggest cross-reactivity with MLF1IP protein from human, dog, bovine and chimpanzee based on 100% homology with the immunizing sequence. Expect partial reactivity with homologues from rat and mouse (90% homology). Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q71F23
• NCBI: 38016935
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-MLF1 pT78 antibody, rabbit anti-MLF1 I
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, western blotting, IF, and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 65 kDa in size corresponding to MLF1IP protein by western blotting in the appropriately stimulated tissue, cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human, multi-brain and multi-cancer slides using Mlf1 (phospho-T78) antibody

Immunohistochemical staining of HeLa cells using Mlf1 (phospho-T78) antibody

Western blot analysis of HeLa cells using Mlf1 (phospho-T78) antibody