MLC1 Antibody

• Catalog Number: orb1248452
• Category: Antibodies
• Description: MLC1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SDSANILDEVPFPAR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 41.1kDa according to NP_055981.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (López-Hernández et al, Hum Mol Genet. 2011 Aug 15;20(16):3266-77.PMID:21624973). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 70kDa
• Target: MLC1
• UniProt ID: Q15049
• NCBI: NP_055981.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: MLC1, megalencephalic leukoencephalopathy with sub
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 41.1kDa according to NP_055981.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (López-Hernández et al, Hum Mol Genet. 2011 Aug 15;20(16):3266-77.PMID:21624973). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248452 (1 ug/ml) staining of Human Hippocampus lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248452 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.