MLC1 Antibody

SKU: orb1248452

Description

MLC1 Antibody

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 70kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 41.1kDa according to NP_055981.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies. Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SDSANILDEVPFPAR
• Target: MLC1
• Molecular Weight: Approx 70kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MLC1, megalencephalic leukoencephalopathy with subcortical cysts 1, LVM, MLC, VL, membrane protein MLC1

orb1248452 (1 ug/ml) staining of Human Hippocampus lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248452 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

MLC1

UniProt

Q15049

RefSeq (Protein):NP_055981.1