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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1:500-1:2000, IHC-P: 1:100-1:300, IF: 1:200-1:1000, ELISA: 1:10000 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from Human MITF around the non-phosphorylation site of S180. |
| Target | MITF |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-MITF antibody, anti-BHLHE32 antibody, anti-Microphthalmia-associated transcription factor antibody, anti-Class E basic helix-loop-helix protein 32 antibody, anti-bHLHe32 antibody
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Western blot analysis of COLO205 cells using MITF antibody
Quick Database Links
Gene Symbol
MITF
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
MITF Antibody (orb671186)
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