MITF Antibody

SKU: orb1410580

Description

Mouse monoclonal antibody to MITF

Research Area

Cardiovascular Research, Immunology & Inflammation, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, IF, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: PCRP-MITF-1D9
• Immunogen: Recombinant full-length human MITF protein
• Target: MITF
• Source/Expression System: Bioreactor
• Molecular Weight: 52-56kDa (doublet)
• Purification: Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BHLHE32; Class E basic helix-loop-helix protein 32 (bHLHe32); CMM8; Mi; Microphthalmia-associated transcription factor; MITF; WS2; WS2A

Analysis of Protein Array containing more than 19000 full-length human proteins using MITF-Monospecific Mouse Monoclonal Antibody (PCRP-MITF-1D9). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Flow cytometric analysis of PFA-fixed HeLa cells. MITF Mouse Monoclonal Antibody (PCRP-MITF-1D9) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow cytometric analysis of PFA-fixed HeLa cells. MITF Mouse Monoclonal Antibody (PCRP-MITF-1D9) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

SDS-PAGE Analysis of Purified MITF Mouse Monoclonal Antibody (PCRP-MITF-1D9). Confirmation of Integrity and Purity of Antibody.

Quick Database Links

Gene Symbol

MITF

UniProt

O75030