MITF Antibody

SKU: orb1925954

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: IHC - 1:1000, WB - 1:2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: B3829EV056X429X69
• Immunogen: This MITF antibody is generated from a mouse immunized with a recombinant protein of human MITF.
• Target: MITF {ECO:0000303|PubMed:8069297, ECO:0000312|HGNC:HGNC:7105}
• Molecular Weight: 58795 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32, MITF, BHLHE32

Immunohistochemical analysis of paraffin-embedded Human Melanoma section using Pink1. Diluted at 1:1000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

All lanes: Anti-MITF Antibody at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: PC-12 whole cell lysate. Lane 3: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

MITF {ECO:0000303|PubMed:8069297, ECO:0000312|HGNC:HGNC:7105}

UniProt

O75030