MITF (Ab-180/73) Antibody

• Catalog Number: orb685570
• Category: Antibodies
• Description: This MITF (Ab-180/73) Antibody is an unconjugated polyclonal product. It tagets MITF using a Synthesized non-phosphopeptide derived from Human MITF around the phosphorylation site of serine 180/73 (P-N-S(p)-P-M) as the immunogen. This antibody is suitable for ELISA, IF, IHC, WB. Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen..
• Target: MITF
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: liquid
• Buffer/Preservatives: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: Synthesized non-phosphopeptide derived from Human MITF around the phosphorylation site of serine 180/73 (P-N-S(p)-P-M).
• UniProt ID: O75030
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: WB: 1: 500-1: 3000, IHC-P: 1: 50-1: 100, IF: 1: 100-1: 500
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-MITF M1 antibody, anti-Microphthalmia-associa
• Research Area: Epigenetics & Chromatin
• Note: For research use only

Western blot analysis of HepG2 cells (Lane 2) and 3T3 cells (Lane 3) using MITF (Ab-180/73) antibody

Immunohistochemical staining of human skin tissue using MITF (Ab-180/73) antibody

Immunofluorescence analysis of HeLa cells using MITF (Ab-180/73) antibody

Western blot analysis of HepG2 cells (lane 1) and COLO205 cells (lane 2) using MITF (Ab-180/73) antibody