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Catalog Number | orb1272363 |
---|---|
Category | Antibodies |
Description | MIP-3a Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-3-alpha (human Macrophage Inflammatory Protein-3-alpha). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | CCL20 |
UniProt ID | P78556 |
NCBI | P78556 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | CKb4, LARC, ST38, MIP3A, MIP-3a, SCYA20, MIP-3-alp Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
To detect Human MIP-3-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human MIP-3-alpha (orb1272362) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-3-alpha.
To detect HumanMIP-3-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect HumanMIP-3-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed paraffin-embedded sections of human colon/rectum adenocarcinoma tissue. The recommended concentration is 0.5ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
IF, IHC-Fr, IHC-P | |
Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC | |
Bovine, Human, Porcine | |
Goat | |
Polyclonal | |
Unconjugated |