CCL20 Rabbit Polyclonal Antibody

• Catalog Number: orb10264
• Category: Antibodies
• Description: CCL20 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CCL20 (31-97/97aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 8.6 kDa
• Target: CCL20
• UniProt ID: P78556
• RRID: AB_10752775
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CCL20_HUMAN; C-C motif chemokine 20; beta-chemokin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Wu, Lili et al. Effects of extracellular magnesium extract on the proliferation and differentiation of human osteoblasts and osteoclasts in coculture Acta Biomater, 27, 294-304 (2015)

PubMed: 26318802

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CCL20) Polyclonal Antibody, Unconjugated (orb10264) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.