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Ccl3 Antibody
SKU: orb1272373
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, NeA, WB |
|---|---|
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-1-alpha (murine Macrophage Inflammatory Protein-1-alpha). |
| Target | Ccl3 |
| Purification | Anti-mMIP-1-alpha specific antibody was purified by affinity chromatography employing immobilized mMIP-1-alpha matrix. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Mip1a, Scya3, G0S19-1, AI323804, MIP1-(a), LD78alpha, MIP-1alpha, MIP1-alpha, Mip1a, C-C motif chemokine 3, Heparin-binding chemotaxis protein, MIP-1-alpha
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To detect Murine MIP-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine MIP-1-alpha (orb1272372) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine MIP-1-alpha.
To detect Murine MIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine MIP-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Murine MIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine MIP-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Ccl3 Antibody (orb1272373)
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