You have no items in your shopping cart.
Page Not Found
Cart summary
Item 1 of 3
Item 1 of 3
MIP-1a Antibody (biotin)
Catalog Number: orb1272675
| Catalog Number | orb1272675 |
|---|---|
| Category | Antibodies |
| Description | MIP-1a Antibody (biotin) |
| Target | CCL3 |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-1a (Human MIP-1 alpha ). Human MIP-1a specific antibody was purified by affinity chromatography and then biotinylated. |
| UniProt ID | P10147 |
| Tested applications | ELISA, WB |
| Application notes | ELISA:Sandwich:To detect hMIP-1a by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human MIP-1a as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1a. Western Blot:To detect hMIP-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | MIP1A, SCYA3, G0S19-1, LD78ALPHA, MIP-1-alpha, MIP Read more... |
| Note | For research use only |
| NCBI | P10147 |
To detect Human MIP-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human MIP-1-alpha (orb1272676) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human MIP-1-alpha.
To detect Human MIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human MIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
- Item 1 of 1
- Item 1 of 1
