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MIP-1a Antibody (biotin)
Catalog Number: orb1272374
| Catalog Number | orb1272374 |
|---|---|
| Category | Antibodies |
| Description | MIP-1a Antibody (biotin) |
| Target | Ccl3 |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Reactivity | Rat |
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat MIP-1-alpha (rat MIP-1-alpha). |
| UniProt ID | P50229 |
| Tested applications | ELISA, WB |
| Application notes | ELISA:Sandwich:To detect Rat MIP-1α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat MIP-1α (XP-5233) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MIP-1α. Western Blot:To detect rat MIP-1-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat MIP-1-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Scya3, MIP-1a, Mip1a, Scya3, C-C motif chemokine 3 Read more... |
| Note | For research use only |
| NCBI | P50229 |
To detect Rat MIP-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Rat MIP-1-alpha (orb1272375) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat MIP-1-alpha.
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