MIF Rabbit Polyclonal Antibody

SKU: orb628810

Description

Rabbit polyclonal antibody to MIF

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: ELISA, IF, IHC, IP, WB
• Dilution Range: WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:50-1:500; IF: 1:50-1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: macrophage migration inhibitory factor(glycosylation-inhibiting factor)
• Target: MIF
• Molecular Weight: 12 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GLIF, MMIF

Y79 cells were subjected to SDS PAGE followed by western blot with orb628810 (MIF antibody) at dilution of 1:1000.

Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using orb628810 (MIF antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG (H+L).

IP Result of anti-MIF (IP: orb628810, 4 ug; Detection: orb628810 1:1000) with mouse spleen tissue lysate 4000 ug.

Immunohistochemistry of paraffin-embedded human testis tissue slide using orb628810 (MIF Antibody) at dilution of 1:200.

Quick Database Links

Gene Symbol

MIF

UniProt

P14174