MIF Antibody

• Catalog Number: orb628810
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MIF
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: macrophage migration inhibitory factor(glycosylation-inhibiting factor)
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:50-1:500; IF: 1:50-1:500
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Conjugation: Unconjugated
• MW: 12 kDa
• Target: MIF
• UniProt ID: P14174
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GLIF, MMIF
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Y79 cells were subjected to SDS PAGE followed by western blot with orb628810 (MIF antibody) at dilution of 1:1000.

Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using orb628810 (MIF antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG (H+L).

IP Result of anti-MIF (IP: orb628810, 4 ug; Detection: orb628810 1:1000) with mouse spleen tissue lysate 4000 ug.

Immunohistochemistry of paraffin-embedded human testis tissue slide using orb628810 (MIF Antibody) at dilution of 1:200.