MIF Antibody

SKU: orb1537918

Description

MIF Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution Range: ELISA, IHC, IHC-P (5 µg/ml), WB (1:500 - 1:2000)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: 4E4
• Immunogen: Recombinant human MIF (1-115 aa) purified from E. coli.
• Target: MIF
• Purification: Protein G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4, 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MIF, GLIF, L-dopachrome tautomerase, L-dopachrome isomerase, MMIF, Phenylpyruvate tautomerase

The extract of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.

Quick Database Links

Gene Symbol

MIF