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Metallothionein Antibody: Biotin
Catalog Number: orb55587
| Catalog Number | orb55587 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody against Metallothionein-1 Conjugated to Biotin |
| Target | Metallothionein |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Biotin |
| Reactivity | Bacteria |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS |
| Immunogen | Synthetic peptide from the mid-protein of Pseudomonas aeruginosa Metallothionein |
| UniProt ID | Q9I1X5 |
| MW | 36 kDa |
| Tested applications | ELISA, ICC, IF, IHC, WB |
| Dilution range | WB (1:1000) |
| Application notes | A 1:1000 dilution of SPC-717 was sufficient for detection of Metallothionein in 15 µg of pseudomonas aeruginosa purified Metallothionein (pmtA-GST) protein by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
| Storage | Conjugated antibodies should be stored according to the product label |
| Alternative names | CES 1 antibody, CES1 antibody, Metallothionein 1A Read more... |
| Note | For research use only |
Western blot analysis of Pseudomonas aeruginosa Purified protein showing detection of ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST) Metallothionein protein using Rabbit Anti-Metallothionein Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Blank. Lane 3: Pseudomonas aeruginosa purified protein with GST tag. Load: 25 μg. Block: 1% milk, 2% BSA. Primary Antibody: Rabbit Anti-Metallothionein Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Mouse Anti-Rabbit IgG: AP at 1:1000 for 60 min at RT. Color Development: Alkaline phosphate activity colorimetric assay. Predicted/Observed Size: ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST).
