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Catalog Number | orb413704 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against Metallothionein-1 Conjugated to Biotin |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 2B5 |
Tested applications | ELISA, ICC, IF, IHC, WB |
Reactivity | Bacteria |
Isotype | IgG1 |
Immunogen | Recombinant full length Pseudomonas aeruginosa Metallothionein protein |
Concentration | 1 mg/ml |
Dilution range | WB (1:1000); ELISA (1:800) |
Conjugation | Biotin |
MW | 36 kDa |
Target | Metallothionein |
Entrez | 880550 |
UniProt ID | Q9I1X5 |
NCBI | NP_250830.1 |
Storage | Conjugated antibodies should be stored according to the product label |
Buffer/Preservatives | 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS |
Alternative names | Metallothionein antibody, CES 1 antibody, CES1 ant Read more... |
Note | For research use only |
Application notes | A 1:1000 dilution of SMC-552 was sufficient for detection of Metallothionein in 1 µg of purified Pseudomonas aeruginosa Metallothionein (PmtA)-GST tagged protein by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |
Western Blot analysis of Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged showing detection of 36 kDa Metallothionein protein using Mouse Anti-Metallothionein Monoclonal Antibody, Clone 2B5. Lane 1: Molecular Weight Ladder (MW). Lane 2: GST. Lane 3: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged. Load: 1 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Metallothionein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 36 kDa.
ELISA, ICC, IF, IHC, WB | |
Bacteria | |
Rabbit | |
Polyclonal | |
Biotin |
ELISA, ICC, IF, IHC, WB | |
Bacteria | |
Mouse | |
Monoclonal | |
Biotin |
ELISA, ICC, IF, IHC, WB | |
Bacteria | |
Mouse | |
Monoclonal | |
Biotin |