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Metallothionein Antibody: Biotin

Catalog Number: orb413704

DispatchUsually dispatched within 2-3 weeks
$ 590.00
Catalog Numberorb413704
CategoryAntibodies
DescriptionMouse monoclonal antibody against Metallothionein-1 Conjugated to Biotin
TargetMetallothionein
ClonalityMonoclonal
Species/HostMouse
IsotypeIgG1
ConjugationBiotin
ReactivityBacteria
Concentration1 mg/ml
Buffer/Preservatives136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
ImmunogenRecombinant full length Pseudomonas aeruginosa Metallothionein protein
UniProt IDQ9I1X5
MW36 kDa
Tested applicationsELISA, ICC, IF, IHC, WB
Dilution rangeWB (1:1000); ELISA (1:800)
Application notesA 1:1000 dilution of SMC-552 was sufficient for detection of Metallothionein in 1 µg of purified Pseudomonas aeruginosa Metallothionein (PmtA)-GST tagged protein by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Clone Number2B5
StorageConjugated antibodies should be stored according to the product label
Alternative namesMetallothionein antibody, CES 1 antibody, CES1 ant
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NoteFor research use only
Entrez880550
NCBINP_250830.1
Metallothionein Antibody: Biotin

Western Blot analysis of Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged showing detection of 36 kDa Metallothionein protein using Mouse Anti-Metallothionein Monoclonal Antibody, Clone 2B5. Lane 1: Molecular Weight Ladder (MW). Lane 2: GST. Lane 3: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged. Load: 1 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Metallothionein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 36 kDa.

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