Metallothionein Antibody: APC

SKU: orb55586

Description

Rabbit polyclonal antibody to Metallothionein-1

Images & Validation

• Tested Applications: ICC, IF, IHC
• Dilution range: WB (1:1000)
• Reactivity: Bacteria
• Application Notes:
  A 1:1000 dilution of SPC-717 was sufficient for detection of Metallothionein in 15 µg of pseudomonas aeruginosa purified Metallothionein (pmtA-GST) protein by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the mid-protein of Pseudomonas aeruginosa Metallothionein
• Target: Metallothionein
• Molecular Weight: 36 kDa
• Purification: Peptide Affinity Purified

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

CES 1 antibody, CES1 antibody, Metallothionein 1A antibody, Metallothionein 1S antibody, Metallothionein 2 antibody, Metallothionein 2A antibody, Metallothionein IA antibody, Metallothionein II antibody, Metallothionein-1A antibody, Metallothionein-IA antibody, Metallothionein2 antibody, MGC32848 antibody, MT 1A antibody, MT 2 antibody, MT 2A antibody, MT IA antibody, MT II antibody, MT-1A antibody, MT-IA antibody, MT1 antibody, MT1A antibody, MT1A_HUMAN antibody, MT1S antibody, MT2 antibody, MT2A antibody, MTC antibody, Probable Metallothionein antibody

Western blot analysis of Pseudomonas aeruginosa Purified protein showing detection of ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST) Metallothionein protein using Rabbit Anti-Metallothionein Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Blank. Lane 3: Pseudomonas aeruginosa purified protein with GST tag. Load: 25 μg. Block: 1% milk, 2% BSA. Primary Antibody: Rabbit Anti-Metallothionein Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Mouse Anti-Rabbit IgG: AP at 1:1000 for 60 min at RT. Color Development: Alkaline phosphate activity colorimetric assay. Predicted/Observed Size: ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST).

Quick Database Links

Gene Symbol

Metallothionein

UniProt

Q9I1X5