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Metallothionein Antibody: APC

SKU: orb413685

Description

Mouse monoclonal antibody to Metallothionein-1

Images & Validation

Tested ApplicationsICC, IF, IHC
Dilution rangeWB (1:1000); ELISA (1:800)
ReactivityBacteria
Application Notes
A 1:1000 dilution of SMC-551 was sufficient for detection of Metallothionein in 1 µg of purified Pseudomonas aeruginosa Metallothionein (PmtA)-GST tagged protein by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.1F5
ImmunogenRecombinant full length Pseudomonas aeruginosa Metallothionein protein
TargetMetallothionein
Molecular Weight36 kDa
PurificationProtein G Purified

Storage & Handling

StorageConjugated antibodies should be stored according to the product label
Buffer/Preservatives95.64mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

Metallothionein antibody, CES 1 antibody, CES1 antibody, Metallothionein 1A antibody, Metallothionein 1S antibody, Metallothionein 2 antibody, Metallothionein 2A antibody, Metallothionein IA antibody, Metallothionein II antibody, Metallothionein-1A antibody, Metallothionein-IA antibody, Metallothionein2 antibody, MGC32848 antibody, MT 1A antibody, MT 2 antibody, MT 2A antibody, MT IA antibody, MT II antibody, MT-1A antibody, MT-IA antibody, MT1 antibody, MT1A antibody, MT1A_HUMAN antibody, MT1S antibody, MT2 antibody, MT2A antibody, MTC antibody, Probable Metallothionein antibody, pmtA antibody

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Metallothionein Antibody: APC

Western Blot analysis of Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged showing detection of 36 kDa Metallothionein protein using Mouse Anti-Metallothionein Monoclonal Antibody, Clone 1F5. Lane 1: Molecular Weight Ladder (MW). Lane 2: GST. Lane 3: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged. Load: 1 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Metallothionein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 36 kDa.

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol

Metallothionein Antibody: APC (orb413685)

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100 μg
$ 660.00
DispatchUsually dispatched within 2-3 weeks
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