MSRA Antibody (N-term)

• Catalog Number: orb1929364
• Category: Antibodies
• Description: MSRA Antibody (N-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q9UJ68
• MW: 26132 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: WB - 1:1000, IF - 1:100, IHC-P - 1:100-500, FC - 1:10-50
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Research Area: Cell Biology, Metabolism Research, Signal Transduc
• Note: For research use only

MSRA Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescent analysis of A549 cells, using MSRA Antibody (N-term). Diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MSRA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of MSRA antibody (N-term) in mouse kidney tissue lysates (35 ug/lane). MSRA (arrow) was detected using the purified Pab.

Anti-MSRA Antibody (N-term) at 1:1000 dilution + human kidney lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.